Influence of polyelectrolytes on increasing sensitivity of an immunofluorescent analysis based on plasmon silver nanoparticles

It was studied the dependence of interaction components immunofluorescence test system for quantitative determination prostate specific antigen based on plasmon silver nanoparticles physicochemical nature various polyelectrolytes used to coat films nanoparticles. has been shown that use a weakly charged polycationic polyelectrolyte poly-L-lysine can increase antigenic binding by 2.34 times, and highly polydiallyldimethylammonium chloride increases affinity prostatic 5 times. When developing immunochemical systems using nanoparticles, an important parameter is choice coating nanolayer, since electrostatic properties significantly affect both sorption capacity solid phase conformational state functional activity immobilized protein molecules. Both specificity sensitivity system, as well minimum possible detectable concentration bioanalyte, largely depend these parameters.